Peer-Reviewed Research

The Science Behind Helios

Photobiomodulation is a peer-reviewed field with over 6,000 published studies. Below are selected studies relevant to the wavelengths used in Helios — sourced directly from PubMed, the U.S. National Library of Medicine.

Important: The studies referenced on this page relate to photobiomodulation (PBM) technology and the biological effects of specific light wavelengths as documented in independent peer-reviewed research. These studies were not conducted on the Helios device and are not intended to imply that Helios diagnoses, treats, cures, or prevents any medical condition. Helios is a consumer oral care device, not a medical device registered for therapeutic claims. Individual results vary. Consult your dentist or healthcare professional before making changes to your oral care routine.

460nm Blue Light

Antimicrobial effect of blue light using Porphyromonas gingivalis pigment

Scientific Reports / PubMed 2017

Yoshida A. et al. — Department of Oral Microbiology, Okayama University, Japan

Researchers confirmed that blue-light irradiation of Porphyromonas gingivalis — the primary bacterium responsible for periodontal disease — is potentially bactericidal through oxidative DNA damage. The study used the bacteria's own intracellular pigment (PpIX) as a photosensitiser, meaning no external chemicals were required. The authors concluded this approach has potential as a treatment for new periodontal diseases and drug-resistant bacteria.

Periodontal pathogens Antibacterial mechanism No photosensitiser required
View on PubMed PMID: 28701797
455nm Blue Light

The effect of blue light on periodontal biofilm growth in vitro

Journal of Periodontal Research / PubMed 2015

Fontana C.R. et al. — School of Dentistry, Boston University, USA

Blue light at 455nm was shown to eliminate key periodontopathogens including P. gingivalis, Prevotella intermedia, P. nigrescens and P. melaninogenica in both pure culture and human dental plaque suspensions. The study also demonstrated efficacy against periodontal biofilms — the form in which bacteria are most resistant to treatment — with bacterial survival reduced by 48.2% following blue-light exposure.

Biofilm reduction Dental plaque Multiple pathogens
View on PubMed PMID: 25759232
660nm Red Light

Photobiomodulation of inflammatory-cytokine-related effects in a 3-D culture model with gingival fibroblasts

Lasers in Medical Science / PubMed 2020

Cardoso L.M. et al. — Universidade Estadual Paulista (UNESP), Brazil

This study used a 3-D gingival fibroblast model to demonstrate that photobiomodulation reduced pro-inflammatory TNF-α synthesis, stimulated VEGF expression, and enhanced cell density in inflamed gum tissue matrices. The authors concluded that PBM can penetrate collagen matrices and stimulate healing in human gingival fibroblasts — the cells directly responsible for gum tissue repair and collagen production.

Gingival fibroblasts Inflammation reduction Tissue repair Collagen matrix
View on PubMed PMID: 32030556
660nm Red Light

Effectiveness of photobiomodulation in the treatment of dentin hypersensitivity after basic periodontal therapy — a randomized clinical trial

Journal of Clinical & Experimental Dentistry / PubMed 2017

López-Jornet P. et al. — University of Barcelona, Spain

This randomised split-mouth clinical trial evaluated 660nm photobiomodulation on 30 patients (120 teeth) with dentin hypersensitivity following scaling and root planing. Results showed that 660nm laser therapy produced statistically significant reductions in sensitivity compared to the control group. The study used identical wavelength parameters to Helios (660nm, continuous mode). The authors noted that diode laser photobiomodulation studies have reported improvement rates of 60–98% for dentin hypersensitivity.

Randomised clinical trial Sensitivity reduction 30 patients 660nm identical wavelength
View on PubMed PMID: 28512549
Multi PBM Review

Effectiveness of photobiomodulation as adjunct to non-surgical periodontal therapy — a systematic review of in vivo human studies

Photodiagnosis and Photodynamic Therapy / PubMed 2020

Systematic review of randomised controlled trials (RCTs)

This systematic review critically appraised published randomised clinical trial data on photobiomodulation therapy (PBMT) as an adjunct to non-surgical periodontal treatment. The review confirmed that PBMT has anti-inflammatory, analgesic, and regenerative properties when applied to periodontal tissue — and that the evidence base from in vivo human RCTs supports its use as a clinically meaningful adjunctive therapy for periodontitis management.

Systematic review Human RCTs only Anti-inflammatory Regenerative
View on PubMed PMID: 33098680
660nm Red Light

Effect of laser photobiomodulation on wound healing and postoperative pain following free gingival graft — a split-mouth triple-blind randomised controlled clinical trial

Lasers in Medical Science / PubMed 2017

Kalhori K.A.M. et al. — Randomised controlled clinical trial, 12 patients

This triple-blind RCT evaluated 660nm photobiomodulation on gingival wound healing. Results demonstrated that 660nm laser therapy produced significantly faster re-epithelialisation of gum tissue at the donor site and meaningfully reduced postoperative pain compared to the control group. This directly supports the mechanism by which 660nm accelerates healing of inflamed or damaged gum tissue.

Triple-blind RCT Gingival healing Pain reduction Re-epithelialisation
View on PubMed PMID: 28549319